Website
Module Guide since WS 2023/24

Modul LS4137-KP09

Bioanalytics C (BioanalC)

Duration:


1 Semester
Turnus of offer:


every summer semester
Credit points:


9
Course of studies, specific field and terms:
  • Master Molecular Life Science 2023 (optional subject), life sciences, 2nd semester
Classes and lectures:
  • LS4139-S: Mass Spectrometry-based Proteomics and Structural Biology (seminar, 0,5 SWS)
  • LS4137-V: Cryo-electron microscopy (lecture, 1 SWS)
  • LS4138-S: Metabolomics (seminar, 1 SWS)
  • LS4139-V: Mass Spectrometry-based Proteomics and Structural Biology (lecture, 1 SWS)
  • LS4138-V: Metabolomics (lecture, 2 SWS)
  • LS4137-S: Cryo-electron microscopy (seminar, 0,5 SWS)
Workload:
  • 105 Hours in-classroom work
  • 165 Hours private studies
Contents of teaching:
  • +++Contents of teaching cryo-EM:+++
  • Common procedures in the cryo-electron microscopy and application to biological systems
  • Anatomy of the cryo-electron microscope
  • Theoretical background: Fourier transforms and image formation
  • Sample preparation
  • Common procedures in the cryo-electron microscopy
  • Single Particle Analysis (SPA) – Background and practical
  • +++Contents of teaching Metabolomics:+++
  • What is Metabolomics, how is it related to metabolism?
  • Underlying analytical techniques: Mass spectrometry and NMR.
  • Use of isotope labeling for metabolic flux analysis.
  • Statistical methods used for Metabolomics.
  • Computational flux-balance modelling.
  • Case studies from current literature (pharmakometabolomics, role of metabolomics in drug research).
  • +++Contents of Practicals Metabolomics:+++
  • Metabolomics: Introduction to practical approaches
  • Using Matlab for data analysis
  • Understanding statistics using practical approaches
  • Computer-based data analysis in Matlab
  • Simple coding for metabolomics
  • +++Contents of teaching Basics of Membrane Biophysics:+++ - see LS4131
Qualification-goals/Competencies:
  • The students understand the methods using in metabolomics research
  • They understand basic metabolic mechanisms and their importance for drug research
  • They understand the statistical methods used in metabolic analyses
  • They have developed a basic understanding of methods used to model metabolic networks
  • Students have a basic theoretical understanding of cryo-EM and can weigh up the method against other structural biology methods
  • The students know which basic experimental steps are required to determine a protein structure.
  • Students have gained initial experience in the processing of cryo-EM data
  • Qualification-goals/Competencies Basics of Membrane Biophysics see LS4131
Grading through:
  • written exam
Responsible for this module:
  • Prof. Dr. rer. nat. Ulrich Günther
Teachers:
  • Prof. Dr. Thomas Krey
  • Prof. Dr. rer. nat. Ulrich Günther
  • PD Dr. rer. nat. Guido Hansen
Literature:
  • new papers:
Language:
  • English, except in case of only German-speaking participants
Notes:

Students who choose the module Bioanalytics C must take the module LS4135 Protein Biophysics in the elective area Biophysics

Prerequisites for the module:
- nothing

Prerequisites for admission to the written examination:
- succesful participation in the exercises

Module exam:
- LS4137-L1: Bioanalytics C, written exam, 90 min, 100 % module grade

Letzte Änderung:
31.3.2025