Duration:
1 Semester | Turnus of offer:
each winter semester | Credit points:
5 |
Course of studies, specific field and terms: - Bachelor Molecular Life Science 2024 (optional subject), life sciences, 5th semester
- Bachelor Biophysics 2016 (optional subject), life sciences, 5th semester
|
Classes and lectures: - Tissue Engineering (seminar with practical exercises, 2 SWS)
- Tissue Engineering (lecture, 2 SWS)
| Workload: - 90 Hours private studies
- 60 Hours in-classroom work
| |
Contents of teaching: | - Lectures: Mamalia cells in their natural environment and under in vitro culture as an example of industrial application.
- Aging of cells in vitro
- Established cell lines
- Diverse in vitro culturing conditions
- Proliferation and differentiation under in vitro conditions
- Stem cell biology
- Materials for medical applications
- Fermentors, bioreactors and protein purification
- Home work e. g. Tissue transplantation and rejection
- Practical course (in groups of 2): Principles of aseptic manipulations, working in sterile containments, object and selfprotection, use of autoclaves
- Preparation of sterile media, additives and other reagents
- Slicing of tissue samples, transfer into tissue culture flasks for explant cultures
- Microscopy and documentation of growing cells
- Cell count, passaging by trypsinisation
- Viability test, freezing of cells and reseeding after thawing
- Adherence of cells to various matrices
- Immunohistochemistry of intracellular and extracellular proteins
| |
Qualification-goals/Competencies: - Students are able to explain principles of cell- and tissue culture to generate biocomposites from differentiated and pluripotent cells
- They are able to explain basic principles of pro- and eukaryotic gene expression systems
- They are able to explain basic principles of matrix biology
- They can reproduce the aspects of stem cell biology
- They acquire the ability to assess ethical aspects of tissue engineering
- They improve their competence for correct documentation and team working skills
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Grading through: |
Responsible for this module: Teachers: - Prof. Dr. rer. nat. Holger Notbohm
- Prof. Dr. Uwe Englisch
- Prof. Dr. rer. nat. Markus Hoffmann, Dr. med.
- Prof. Dr. rer. nat. Charli Kruse
- Dr. C. Probst
- Dr. rer. nat. Daniel Hans Rapoport
- Prof. Dr. med. vet. Jennifer Hundt
- Prof. Dr. med. Ralf Ludwig
- Dr. rer. nat. Olaf Isken
|
Literature: - Lanza, Langer, Vacanti: Principles of Tissue Engineering
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Language: |
Notes:Knowledge in Cell biology is a prerequisite for this course. Entrance requirement for the practical course: certificate of the course Biochemistry 1 or 2. |
Letzte Änderung: 2.7.2024 |
für die Ukraine