Modul LS3251-KP05

Tissue Engineering (TissueEng)

Duration:

1 Semester

Turnus of offer:

each winter semester

Credit points:

Course of studies, specific field and terms:

Bachelor Molecular Life Science 2024 (optional subject), life sciences, 5th semester
Bachelor Biophysics 2016 (optional subject), life sciences, 5th semester

Classes and lectures:

Tissue Engineering (seminar with practical exercises, 2 SWS)
Tissue Engineering (lecture, 2 SWS)

Workload:

60 Hours in-classroom work
90 Hours private studies

Contents of teaching:

Lectures: Mamalia cells in their natural environment and under in vitro culture as an example of industrial application.
Aging of cells in vitro
Established cell lines
Diverse in vitro culturing conditions
Proliferation and differentiation under in vitro conditions
Stem cell biology
Materials for medical applications an biotechnology
Fermentors, bioreactors and protein purification
Home work e. g. Tissue transplantation and rejection
Practical course (in groups of 2): Principles of aseptic manipulations, working in sterile containments, object and selfprotection, use of autoclaves
Preparation of sterile media, additives and other reagents
Slicing of tissue samples, transfer into tissue culture flasks for explant cultures
Microscopy and documentation of growing cells
Cell count, passaging by trypsinisation
Viability test, freezing of cells and reseeding after thawing
Adherence of cells to various matrices
Immunohistochemistry of intracellular and extracellular proteins

Qualification-goals/Competencies:

Students are able to explain principles of cell- and tissue culture to generate biocomposites from differentiated and pluripotent cells
They are able to explain basic principles of pro- and eukaryotic gene expression systems
They are able to explain basic principles of organoid biology
They can reproduce the aspects of stem cell biology
They acquire the ability to assess ethical aspects of tissue engineering
They improve their competence for correct documentation and team working skills

Grading through:

written exam

Responsible for this module:

Prof. Dr. rer. nat. Charli Kruse

Teachers:

Prof. Dr. rer. nat. Charli Kruse
Dr. rer. nat. Daniel Hans Rapoport
Dr. rer. nat. Philipp Ciba
Prof. Dr. med. vet. Jennifer Hundt
Prof. Dr. med. Ralf Ludwig
Dr. rer. nat. Olaf Isken
Dr. med. Dipl. Biol. Judith Sewing

Literature:

Lanza, Langer, Vacanti: Principles of Tissue Engineering

Language:

offered only in German

Notes:

Knowledge in Cell biology is a prerequisite for this course. Entrance requirement for the practical course: certificate of the course Biochemistry 1 or 2.

Letzte Änderung:

8.10.2024