Module LS4020 D

Module part LS4020D: Microscopy: Techniques and Applications (StrAnaMikr)

Duration:

1 Semester

Turnus of offer:

each winter semester

Credit points:

Course of studies, specific field and terms:

Master CLS 2023 (Module part of a compulsory module), MML with specialization in Life Science, 3rd semester
Master MLS 2018 (Module part of a compulsory module), structure biology, 1st semester
Master Infection Biology 2018 (Module part of a compulsory module), Interdisciplinary modules, 1st semester
Master Biophysics 2019 (Module part of a compulsory module), biophysics, 1st semester
Master CLS 2016 (Module part of a compulsory module), MML with specialization in Life Science, 3rd semester
Master MLS 2016 (Module part of a compulsory module), structure biology, 1st semester
Master Infection Biology 2012 (Module part of a compulsory module), Interdisciplinary modules, 1st semester
Master CLS 2010 (module part), computational life science / life sciences, 3rd semester

Classes and lectures:

LS4027-V Optical Methods (lecture, 2 SWS)

Workload:

30 Hours in-classroom work
60 Hours private studies

Contents of teaching:

Basic principles of optics
Light sources and detectors
Classical light microscopy
Photophysics, fluorescence microscopy
Confocal microscopy
Nonlinear microscopy
Fluorescent dyes; GFP and genetically encoded fluorescent markers; live cell/intravital imaging: important experimental parameters
Protein-protein interactions in living cells: FRET, FLIM; biosensors
Photoactivatable/switchable fluorescent proteins; fluorescent timers
Optogenetics: Cell manipulation by light
Super-resolution 3D fluorescence microscopy: STED, PALM, STORM
Optical tweezers as instrument for nanomanipulation
Visualization and quantitative evaluation; data format and data storage media
In vivo imaging in tissues and living animals
Bioluminescence and optoacoustic imaging
Flow cytometry & fluorescence activated cell sorting
High-content screening; optical sensor technology
Technologies under development

Qualification-goals/Competencies:

Students acquire professional competence in basic principles and concepts of optics.
Students know the basics of light and fluorescence microscopy.
They know and understand the most important methods for marking and microscopic visualization of proteins and sub-cellular structures.
Students know the possible applications of live cell microscopy, intravital imaging, and quantitative fluorescence techniques in biological questions.
They know basic techniques of 3-dimensional optical imaging of tissues and animals.
Student are familiar with current research topics in the field of optical methods in the life sciences and are able to evaluate them in terms of their application maturity and potential.
Students can classify optical methods according to their complexity and outline possible applications.
The students have the social and communication skills to discuss given questions within group work for lecture preparation and lecture follow-up.

Grading through:

written exam

Responsible for this module:

Siehe Hauptmodul

Teachers:

Institute of Biomedical Optics

Prof. Dr. rer. nat. Gereon Hüttmann
Prof. Dr. rer. nat. Sebastian Karpf
Dr. rer. nat. Norbert Linz
Prof. Dr. rer. nat. Robert Huber

Literature:

J. B. Pawley, ed.: Handbook of Biological Confocal Microscopy, Springer
V. V. Tučin: Handbook of optical biomedical diagnostics, SPIE Press
L. V. Wang, and H.-i. Wu: Biomedical optics principles and imaging, Wiley
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Language:

offered only in English

Notes:

Is module part of:
- LS4021-KP06 (former LS4020-IB) -> Prof. Hübner
- LS4020-KP06 (former LS4020-MLS) and LS4020-KP12 -> Prof. Peters
- LS4026-KP06 start 2023

(Share of Institute of Biomedical Optics to this lecture is 100%)

Letzte Änderung:

23.9.2024