Modul unbenutztLS5200 SI

Module part: Characterization of Protein Complexes in Structural Biology (ProtCompl)

1 Semester

each winter semester

Master MLS 2009 (Module part of a compulsory module), advanced curriculum MLS, 3rd semester
Master Infection Biology 2012 (Module part of a compulsory module), Interdisciplinary internships and consolidation courses, 3rd semester

Characterization of Protein Complexes in Structural Biology (Seminar and practical course, 2 SWS)

Contents of teaching:
Protein complex formation as the basis of all biochemical cascade Basic techniques to study protein complexes. Theories to be covered: Fluorescence Resonance Transfer (FRET)/ Isothermal Calorimetry (ITC)/ Surface Plasmon Resonance (SPR)/ Circular Dichroism (CD) Experimental techniques to be covered: Protein Complex Preparation/ FRET and SPR

This module aims to address at expanding the knowledge of biophysical and biochemical characterization methods of protein complexes, which is the basis of all working enzymatic cascades. The module will include lectures and hands-on practical sessions. We will also visit CSSB/DESY
You will learn:
How to prepare a protein complex
How to label proteins with fluorescent dye for single molecule fluorescence measurements
UV / Vis spectra and discussions thereof
How to setup the experiments to determine dissociation constant of the complex formation
Different method for determination of complex formation (FRET, SPR)
Discussion of the obtained results and comparisons of the results with other available structural data.

For MLS and IB.
Requirements: All modules of the 1st and 2nd semesters MSc Infection Biology must be passed.

29.7.2016